The module broadly aims to cover the application of genetics in modern biology. The content will include aspects of classical genetics developed beyond that of level 1 and the major techniques of genetic engineering as a support to level 3 modules where the applications will be dealt with in detail. From studying the molecular structure of genes (a comparison between prokaryotes and eukaryotes - the mosaic structure of eukaryotic genes, regulatory elements, promoters, terminators) and basic genome structure, the basic, classic strategies for cloning will be considered. The tools for genetic engineering restriction enzymes, DNA ligase, vectors and gene libraries, their construction - genomic and cDNA libraries will be covered followed by the various methods of selecting individual genes from libraries. Nucleic acid hybridisation (Northern and Southern blotting), DNA sequencing and the polymerase chain reaction will be covered as major techniques in DNA technology. Overall the content consists of some of the fundamental techniques of molecular biology related to DNA analysis.

Lectures/workshops (12 x 2 hrs) will provide guidance to the major topics of the course.

Independent Study (126 hours). Core material will be managed by a student-centred approach - guided reading and work from the recommended book. Videos and the internet will provide some information. Handouts developing self-directed study will be used, possibly along with computer-based material. Wide use of problem-based learning will used. The time will also be used to prepare assignments.

Brown, T. A. 2002. Gene Cloning and DNA Analysis. 4th ed.
A.J.F.Griffiths, W.M. Gelbart, J.H. Miller and R.C. Lewontin (1999) Modern Genetic Analysis. W.H.Freeman . This has an associated CD-ROM.

A single text does not readily support this module.
Video material is also available in the School Learning resource Centre.
On the local server are two cd -roms highly relevant to the course -

The Roche Genetics Educational Program - the first section "Introduction to Genetics" is important introductory material.
PCCAL "Introduction to Recombinant DNA Technology (v2.0). This is full of relevant important material to which you will be directed.

The internet is a very important resource for this module and the students will be encouraged to make wide use of it.

Lecture room with equipment for PC projection, use of video material. Access to library, computers.

Study of
SHS80105-1 Genes & Genomes or
SCS80119-1 Fundamental Molecular Biology

Students will be required to complete 2 pieces of summative assessment.
50% 1-hour In-class test largely based on data analysis (1 - 4)
50% 2000-word evaluation of published research (1 - 3, 5)

Students will be provided with formative assessment and feedback via tasks given at the end of the lectures and reviewed in later sessions.