Module Descriptors
DNA ANALYSIS
BIOL50478
Key Facts
School of Health, Science and Wellbeing
Level 5
15 credits
Contact
Leader: Harry Mountain
Email: H.A.Mountain@staffs.ac.uk
Hours of Study
Scheduled Learning and Teaching Activities: 36
Independent Study Hours: 114
Total Learning Hours: 150
Assessment
- LABORATORY LOG BOOK weighted at 50%
- EXAMINATION - UNSEEN IN EXAMINATION CONDITIONS weighted at 50%
Module Details
Module Special Admissions Requirements
Study of SHS80100-1 Bioskills
Module Resources
Laboratories and relevant equipment. Technical support. Lecture theatre, OHP and PowerPoint projector. Video for tutorial material.
Module Learning Strategies
The module will be delivered as a programme of six four-hour practical classes and twelve one hour lectures. These will be a Workshops will form part of the practical classes. Independent study will be used to introduce the students to the background theory of the techniques, and for data analysis and report writing.
Module Additional Assessment Details
Students will be required to complete two pieces of summative assessment.
1. Laboratory book and report (2000 words) with analysis, interpretation and discussion of practical work. Weighting 50%. (1, 2, 3 & 4).
2. 2 hour exam. Weighting 50%. (3 & 5).
Students will be provided with formative assessment and feedback.
Students will be asked to complete worksheets as part of their independent study. These will form the basis of discussion in the workshops.
1. Laboratory book and report (2000 words) with analysis, interpretation and discussion of practical work. Weighting 50%. (1, 2, 3 & 4).
2. 2 hour exam. Weighting 50%. (3 & 5).
Students will be provided with formative assessment and feedback.
Students will be asked to complete worksheets as part of their independent study. These will form the basis of discussion in the workshops.
Module Indicative Content
Bioinformatics underpins many fast moving, important fields of molecular biology. In this module the technology behind it will be examined, the rationale and motivation behind the numerous large scale genome sequencing projects, the progress to date, the organisation and structure of genomes, genome projects completed to date.
The module will focus on the principles and the applications of molecular biology for studying DNA and generating sequence information. The module gives practical experience of the techniques widely used in research and which generate the raw data which is analysed through the application of bioinformatics
The practical content includes some basic techniques of molecular biology related to DNA analysis and genotyping, including purification and gel electrophoresis of nucleic acids, hybridisation technology, polymerase chain reaction and DNA sequencing. What information can be gleaned from genetic data?
The lectures build on genetics from level 1 and earlier in level 2, focussing on genomics - the structure of genomes and genes and the application of this information in gene identification and analysis of genomic sequences.
The module will focus on the principles and the applications of molecular biology for studying DNA and generating sequence information. The module gives practical experience of the techniques widely used in research and which generate the raw data which is analysed through the application of bioinformatics
The practical content includes some basic techniques of molecular biology related to DNA analysis and genotyping, including purification and gel electrophoresis of nucleic acids, hybridisation technology, polymerase chain reaction and DNA sequencing. What information can be gleaned from genetic data?
The lectures build on genetics from level 1 and earlier in level 2, focussing on genomics - the structure of genomes and genes and the application of this information in gene identification and analysis of genomic sequences.
Module Texts
A single text does not readily support this module. Introductory material for the practicals will be produced by the teaching team. General genetics and books will support the theory content.
Section of the books below are relevant.
S. B. Primrose & R.M Twyman (2003). "Principles of Genome Analysis 3rd edition" Blackwell Science. ISBN 1-40510-120-2.
S. B. Primrose & R.M Twyman (2004). Genomics: Applications in Human Biology. Blackwell Publishing. ISBN 1-4051-0819-3.
G. Gibson & S.V. Muse (2004). A primer of genomic science, 2nd edition. Sinauer. ISBN 0-87893-232-1.
You may also find the following texts useful
T.A. Brown (2002) Genomes 3d ed. Garland Science. ISBN 978-0815341383
C.Dennis & R. Gallagher (2001). The Human Genome. Palgrave. ISBN 0-333-97143-4.
The internet is a very important resource for this module and the students will have to make wide use of it. Sites are numerous but the following will be important
NCBI at http://www.ncbi.nlm.nih.gov/
ExPasy at http://expasy.org/
GeneMark at http://exon.gatech.edu/GeneMark/
J.Craig Ventor Institute at http://www.tigr.org/
GeneWeb at http://bioweb.uwlax.edu/GenWeb/genweb.html
Section of the books below are relevant.
S. B. Primrose & R.M Twyman (2003). "Principles of Genome Analysis 3rd edition" Blackwell Science. ISBN 1-40510-120-2.
S. B. Primrose & R.M Twyman (2004). Genomics: Applications in Human Biology. Blackwell Publishing. ISBN 1-4051-0819-3.
G. Gibson & S.V. Muse (2004). A primer of genomic science, 2nd edition. Sinauer. ISBN 0-87893-232-1.
You may also find the following texts useful
T.A. Brown (2002) Genomes 3d ed. Garland Science. ISBN 978-0815341383
C.Dennis & R. Gallagher (2001). The Human Genome. Palgrave. ISBN 0-333-97143-4.
The internet is a very important resource for this module and the students will have to make wide use of it. Sites are numerous but the following will be important
NCBI at http://www.ncbi.nlm.nih.gov/
ExPasy at http://expasy.org/
GeneMark at http://exon.gatech.edu/GeneMark/
J.Craig Ventor Institute at http://www.tigr.org/
GeneWeb at http://bioweb.uwlax.edu/GenWeb/genweb.html